TY  - JOUR
UR  - http://doi.org/10.1080/10408398.2025.2566387
AV  - none
N2  - Understanding how dietary compounds affect human health is challenged by their molecular complexity and cell-type?specific effects. Conventional multi-cell type (bulk) analyses obscure cellular heterogeneity, while animal and standard in vitro models often fail to replicate human physiology. Single-cell omics technologies?such as single-cell RNA sequencing, as well as single-cell?resolved proteomic and metabolomic approaches?enable high-resolution investigation of nutrient?cell interactions and reveal mechanisms at a single-cell resolution. When combined with advanced human-derived in vitro systems like organoids and organ-on-chip platforms, they support mechanistic studies in physiologically relevant contexts. This review outlines emerging applications of single-cell omics in nutrition research, emphasizing their potential to uncover cell-specific dietary responses, identify nutrient-sensitive pathways, and capture interindividual variability. It also discusses key challenges?including technical limitations, model selection, and institutional biases?and identifies strategic directions to facilitate broader adoption in the field. Collectively, single-cell omics offer a transformative framework to advance human-centric nutrition research.
JF  - Critical Reviews in Food Science and Nutrition
KW  - Nutrition research; organoids; personalized nutrition; 3Rs; single-cell omics
ID  - uniromana17867
EP  - 15
SP  - 1
SN  - 1040-8398
TI  - Single-cell omics for nutrition research: an emerging opportunity for human-centric investigations
Y1  - 2025/11//
A1  - Cassotta, Manuela
A1  - Armas Diaz, Yasmany
A1  - Cianciosi, Danila
A1  - Yang, Bei
A1  - Qi, Zexiu
A1  - Chen, Ge
A1  - Gracia Villar, Santos
A1  - Dzul López, Luis Alonso
A1  - Grosso, Giuseppe
A1  - Quiles, José L.
A1  - Xiao, Jianbo
A1  - Battino, Maurizio
A1  - Giampieri, Francesca
ER  -